Impact of oligodendrocyte secreted factors on hippocampal GABAergic neurons: an electrophysiological and transcriptomic study

In the present work, we gain insight into the impact of oligodendrocyte secreted factors on hippocampal GABAergic neuron physiology, and performed patch-clamp recordings followed by single-cell RNA sequencing of neurons. Our results demonstrate that the GABAergic neuron excitability is influenced by glial cells and oligodendrocyte-secreted factors. Moreover, we show specific ion channels alterations and find a correlation with electrophysiological parameters suggesting possible mechanisms for the OCM-induced regulation of neuronal function.本研究旨在探究少突胶质细胞(oligodendrocyte)分泌因子对海马γ-氨基丁酸能神经元(hippocampal GABAergic neuron)生理特性的影响，我们对神经元完成膜片钳记录(patch-clamp recordings)后，进一步开展了单细胞RNA测序(single-cell RNA sequencing)。研究结果显示，γ-氨基丁酸能神经元的兴奋性受到神经胶质细胞(glial cells)与少突胶质细胞分泌因子的调控。此外，我们观测到特定离子通道(ion channels)的改变，并发现其与电生理参数(electrophysiological parameters)存在相关性，这为OCM介导的神经元功能调控机制提供了潜在线索。