Venous plexus-associated lymphoid hubs support meningeal humoral immunity

We have identified previously unappreciated dural-associated lymphoid tissue hubs, present in homeostasis, and anatomically poised to acquire local and systemic antigens. These venolymphatic hubs facilitate rapid expansion of antigen specific immune responses and likely help defend the underlying CNS parenchyma against foreign invaders. BCR sequencing of anatomical/immune compartments 1 week or 3 weeks following nasal VSV challenge, or in naïve mice. Sequencing was performed on paired skull base, rostral-rhinal hub, superior saggital sinus dura, skull bone marrow, cervical lymph node and spleen tissues. Anti-LFA1/VLA4 antibodies or isotype control antibodies were administered starting 6 days post infection (6dpi) where indicated.