Development of embryos cloned from cells of three different Yasufuku cell lines.

*Fusion was examined one hour after electrofusion of couplets of cells and enucleated oocytes. Percentage of the number of the couplets.
¶Blastocyst development was examined 168 h after nuclear transfer. Percentage of the number of cultured embryos.
†Single blastocysts were transferred into single synchronized recipients. Several blastocysts were stored in liquid nitrogen by a vitrification technique. Ten vitrified and warmed embryos were transferred to recipients.
‡Pregnancy was examined by ultrasonography at 33 days after embryo transfer. Percentage of the number of transferred recipients.
aOne recipient delivered a healthy male calf on 30 November 2007. One delivered a mummified fetus on 27 August 2007. One recipient that received a vitrified embryo delivered a healthy male calf on 5 March 2008 but the calf died two days after birth.
bTwo recipients, which received vitrified embryos delivered two healthy male calves on 22 and 31 July 2008.