RNA-Seq analysis of SRSF3 KO hearts compared to controls and iCLIP analysis of SRSF3 binding profile in neonatal cardiomyocytes

The goal of this study is to analyse the transcriptome of control hearts vs hearts lacking SRSF3 expression and to analyse binding preferences of SRSF3 in cardiac myocytes. Overall design: SRSF3 KO and control mice were treated with 3 inyection of 1 mg 4-hydroxytamoxifen in non-consequtive days. Hearts were extracted 5 days after the last inyection. Three different hearts were included for each condition. Neonatal cardiomyocytes were extracted from C57BL/6 mice at P0 or P1.23 A total of 8 million cells were plated in 10 cm2 plates. The following day, cells were transfected with 15 µg of SRSF3-GFP modRNA using 21 µL Lipofectamine 2000. At 48 h post-transfection, cells were irradiated once with 100 mJ UV light (254 nm), and iCLIP was performed