MOESM4 of Gene copy number and function of the APL1 immune factor changed during Anopheles evolution

Additional file 4: Alignment S1. FASTA-format sequence data file for manual validation of single APL1 gene in Anopheles stephensi. By chromosome synteny, the Anopheles stephensi intergenic region between the APL1 gene (ASTE016290 in the SDA-500 assembly) and the adjacent gene (ASTE008334) would contain other APL1 family paralogs, if other APL1 genes exist in An. stephensi. The ~ 7 kb intergenic region contains unjoined sequences in the SDA-500 assembly and thus the true size was unknown. The region was amplified and Sanger sequenced by the strategy indicated in Additional file 1: Figure S1 and amplicons were assembled as a single contig, resolving the region between primer ST11 and ST25 (Additional file 3: Table S1). The resolved sequence does not contain sequences related to APL1, confirming the absence of APL1 paralogs in An. stephensi.