A polymorphic intronic enhancer at 19q12 drives CCNE1 expression in bladder cancer cells. Homo sapiens

We performed chromatin immunoprecipitation against a marker of active regulatory elements in bladder cancer cell lines to highlight regulatory regions specific to the bladder cancer genome. We then compared our dataset to other publicly available datasets to identify any novel, bladder-specific regulatory regions that also overlap with bladder-associated inherited polymorphisms. We used this procedure to determine that there is an intronic enhancer within the CCNE1 gene that binds KLF5 and modulates CCNE1 expression. Overall design: Examination of 1 histone modification (H3K27ac) in 3 different bladder cancer cell lines