Summary of newly confirmed sRNAs in chromosome and virulence plasmid of Shigella flexneri strain 301.

aThe middle arrow represents the sRNA gene, while the flanking arrows indicate the orientation of the adjacent genes, respectively. Genes present on the strand given in the S. flexneri strain 301 genome database are indicated by (→), and genes present on the complementary strand are indicated by (←).bThe sRNA 3′ boundaries are from rho-independent terminator predictions. 5′ boundaries are calculated according to the 3′-ends and northern results.csRNAs were predicted based on different methods. R, RNAz prediction; M, tiling array hybridization; P: RT-PCR verification.