Dose dependent reprogramming of neutrophils by LPS

We have performed analyses of murine primary bone marrow derived neutrophils challenged with either ultra-low dose or high dose of LPS. Neutrophils can be differentially programmed to distinct states by varying dosages of LPS. Purified bone marrow neutrophils were treated with PBS, 100 pg/ml LPS or 100 ng/ml LPS overnight, and harvested for scRNAseq analysis to examine their profiles of gene expression. Overall design: WT murine neutrophils were treated with either PBS, 100pg/ml LPS or 100 ng/ml LPS overnight