EPR (BC030870) ChIRP-Seq analysis in NMuMG mouse mammary gland

EPR, a lncRNA present in the chromatin fraction of NMuMG cells, controls proliferation and fate determination in mammary gland cells. We wanted to identify its genomic targets. We identified thousands of targets, many of them located in regulatory regions. Chromatin Isolation by RNA Purification (ChIRP) was performed according to the protocol published by Chu et al. (Mol.Cell 44, 667–678, 2011) in exponentially growing NMuMG cells. Two distinct pools (EVEN and ODD) of bio-TEG primers were utilized.