<b>The genome annotation files for </b><b>the predatory flower bug </b><b><i>Orius strigicollis</i></b>
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Predatory insects serve as important biological control agents in ecosystems by efficiently reducing pest populations. <i>Orius strigicollis</i> is a highly effective beneficial predator in cultivated fields in China. It is widely used as a biological control agent against many pests, including thrips, aphids, and spider mites. The lack of public availability of <i>O. strigicollis</i> genomes has limited our ability to thoroughly explore and research its evolution and gene function, particularly genes related to pesticide resistance.<i> </i>Here, we report a chromosome-scale assembly of the <i>O. strigicollis</i> genome by PacBio High fidelity (HiFi) long-reads, high-throughput chromosome conformation capture (Hi-C) and Illumina sequencing technologies. The final genome assembly was 167 Mb with a contig N50 size of 12.05 Mb, and 98.21% of the assembly was anchored to 12 chromosomes. Annotation identified 11,551 protein-coding genes. In this article, we reported a high-quality chromosome-scale assembly genome for the species of the genus <i>Orius</i> for the first time, which provide valuable resources for functional gene research and genetic transformation of natural enemy insects.



