Effect of serum extracellular vesicle depletion protocols on miRNA expression of cultured cells. Homo sapiens

This study examined microRNA expression of cells maintained in media prepared with replete serum (EVR) or with serum depleted of extracellular vesicles (EVs) by either of two methods: standard overnight ultracentrifugation (UC-EVD) or a proprietary method used by Thermo Fisher (TF-EVD). Overall design: Human donor monocyte-derived macrophages, T-lymphocytic ACH-2 cells (HIV latency model), and promonocytic U1 cells (HIV-latency model) were maintained in the respective media, with the harvested media conditioned for 48 hours before harvesting. Total RNA was extracted, and miRNA profiles were generated for each sample by custom TaqMan low-density array (TLDA, Thermo Fisher).