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On the risk of manual annotations in 3D confocal microscopy image segmentation

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This dataset contains different annotated masks of human induced pluripotent stem cell nuclei from the dataset published with https://doi.org/10.1038/s41586-022-05563-7 and DL models trained using these masks. Napari-GT and Slicer-GT were manually annotated using the Napari and 3D Slicer software considering only the DNA channel, while for bioGT the Lamin B1 channel was annotated using the seeded watershed algorithm to obtain a reproducible and biologically plausible nucleus annotation. This dataset is provided to reproduce the results in the manuscript "On the risk of manual annotations in 3D confocal microscopy image segmentation", more details can be found there.

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2023-08-21
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