Supplemental Material for Salim et al., 2021

Figure S1 contains additional validation of the qRIN assay. Figure S2 contains analysis of rDNA stability in <i>fob1</i>D mutants. Figure S3 contains analysis of the loss of MATALPHA-LEU2 in <i>fob1</i>D mutants. Figure S4 contains analysis of rDNA stability in nicotinamide. Figure S5 contains validation of the qRIN assay for the <i>CUP1-MAT</i><i>a</i> reporter strains. Figure S6 contains validation of hits with altered rDNA stability. Figure S7 contains functional validation and additional supporting information with regard to deletion of <i>CUP2</i> in <i>CUP1-MAT</i><i>a</i> reporter strains. Figure S8 contains stress and locus-specific effects of copper treatment. Figure S9 contains data regarding how deletion of <i>RTT109, HST3, and HST4</i> affect stability of <i>TUB1</i>. Figure S10 contains data regarding how H3K56 acetylation restricts transcription-induced amplification of the <i>CUP1</i> array in a 17 copy strain. File S1 describes how to calculate repeat loss rates. File S2 describes the method for determining and the location of the <i>MAT</i><i>a</i><i>-LEU2</i> casette in reporter strains. Table S1 summarizes rDNA and <i>CUP1</i> instability measurements from the literature. Table S2 is a list of yeast strains. Table S3 is a list of primers. Table S4 contains validation copy number measurements for 25S, <i>CUP1</i>, <i>LEU2</i> and <i>MATALPHA1</i> in various yeast strains<i>.</i> Table S5 contains the rDNA repeat loss rates and copy number measurements from the screens. Table S6 contains rDNA copy number measurements from the temperature sensitive mutant collection. Table S7 contains copy number measurements of subcultured <i>CUP1-MAT</i><i>a</i> strains.