Linking water to the bottom: eDNA study of benthic invertebrates and invasive species in the Venice lagoon

This study investigates the benthic invertebrate community in the Venice Lagoon using environmental DNA (eDNA) metabarcoding based on superficial water samples. Our objective is to understand if, in a shallow lagoon system, sampling at the surface can provide information on benthic biodiversity, allowing us to establish a baseline for future assessments and to monitor the community's seasonal and spatial variation. eDNA was collected from surface water samples at two sites during nine sampling campaigns from November 2018 to October 2019, and metabarcoding was performed using an available primer pair targeting 16S mitochondrial rDNA of echinoderms, never tested empirically before. Analyses revealed 80 taxonomic units, five not assigned at the species level, belonging not only to the phylum Echinodermata but predominantly assigned to Mollusca, with the majority of the identified species (60 out of 75) representing benthic invertebrates. Several species known to be invasive were detected,..., , # Data from: Linking water to the bottom: eDNA study of benthic invertebrates and invasive species in the Venice lagoon

This submission contains ancillary information for the paper \"Linking Water to the Bottom: eDNA Study of Benthic Invertebrates and Invasive Species in the Venice Lagoon\" (Environmental DNA, Print ISSN: 2637-4943, Online ISSN: 2637-4943, Article ID: EDN370093).

It contains:

1. A csv file (\"metadata_LinkingWaterToTheBottom.csv\") containing the full metadata needed to demultiplex the raw data deposited in the NCBI SRA Archive (BioProject accession number: PRJNA1208720). The file contains the Filter ID used in the original paper, the sample-id that identifies for each filter the three PCR replicates made (pooled in the original paper), the collection location, the date of collection, the filter type, information on controls the sequences of the barcodes and primer used; n/a indicates not \"not applicable\".
2. A text file (\"Pipeline_LinkingWaterToTheBottom.txt\") containin...,