Giant panda picorna-like virus Metagenome

During 2016, to investigate the virome of the fecal sample of the wild giant panda in the Qinling Mountains, China. The sample was collected by disposable materials and re-suspended in ten volumes of phosphate-buffered saline and vigorously vortexed for 5 min. The supernatant was then collected after centrifugation. 500 uL of supernatant was filtered through a 0.45-mm filter (Millipore) to remove eukaryotic- and bacterial cell-sized particles. The filtrate enriched in viral particles was treated with DNase and RNase to digest unprotected nucleic acid for 60 min. Remaining total nucleic acid was then isolated using QiaAmp Mini Viral RNA kit according to manufacturer's protocol. The library was then constructed using Nextera XT DNA Sample Preparation Kit (Illumina) and sequenced using the MiSeq Illumina platform with 250 bases paired ends with dual barcoding for each pool. For bioinformatics analysis, paired end reads of 250 bp generated by MiSeq Illumina platform were debarcoded using vendor software from Illumina.