Data from: Multi-scale structure of chromatin condensates explains phase separation and material properties

The structure and interaction networks of molecules within biomolecular
condensates are poorly understood. Using cryo-electron tomography and
molecular dynamics simulations, we elucidated the structure of phase
separated chromatin condensates across scales, from individual amino acids
to network architecture. We found that internucleosomal DNA linker length
controls nucleosome arrangement and histone tail interactions, shaping the
structure of individual chromatin molecules within and outside
condensates. This structural modulation determines the balance between
intra- and intermolecular interactions, which governs the molecular
network, thermodynamic stability, and material properties of chromatin
condensates. Mammalian nuclei contain dense clusters of nucleosomes whose
non-random organization is mirrored by the reconstituted condensates. Our
work explains how the structure of individual chromatin molecules
determines physical properties of chromatin condensates and cellular
chromatin organization.