Reproducibility of detecting differences in dystrophin expression in muscle biopsies with a wide range of dystrophin levels from healthy controls to BMD and DMD samples by immunofluorescence using MANDYS106 antibody and Definiens intensity measurements (at ‘control’ microscope settings).
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The intra-assay precision (CV%) was calculated for the mean dystrophin intensity of multiple sections of a biopsy/sample analyzed in the same experiment. Inter-assay precision between experiments performed on different days was assessed in two ways by ranking of the samples based on the dystrophin intensity in each experiment and by calculating the CV% from the mean dystrophin intensity in each experiment (experiment 1 and 2 performed 102 days apart). BMD, Becker muscular dystrophy; CV, coefficient of variation; DMD, Duchenne muscular dystrophy; SD, standard deviation. Reproducibility of detecting differences in dystrophin expression in muscle biopsies with a wide range of dystrophin levels from healthy controls to BMD and DMD samples by immunofluorescence using MANDYS106 antibody and Definiens intensity measurements (at ‘control’ microscope settings).
