Comparitive transcriptome analysis of two zebrafish mutants goosepimples/myovb and romeharsha. Comparitive transcriptome analysis of two zebrafish mutants goosepimples/myovb and romeharsha

This dataset comprises RNA-Seq data from two zebrafish mutants and their respective sibling controls. These mutants show trafficking defects leading to accumuation of early and late endocytic vesicles and rounding up in the periderm. The goosepimples mutation has been identified as a null form of MyoVb, an actin based motor. Differential expression analysis suggests upregulation of grainyhead familiy transcription factors Grhl1 and Grhl3 along with various adhesion associated genes. Overall design: RNA was extracted from 20 representative mutant and sibling embryos out of three clutches per mutant (goosepimples siblings, goosepimples mutants, romeharsha siblings, romeharsha mutants) at 48 hours post fertilization cDNA was prepared and sequencing was performed on Illumina Hi seq 2000