Loss of ATM and expression of E4orf4 accelerate Ad infection.

(A) A-T cells and matching WT control cells were infected with the Ad mutants dl366* and dl366*+E4orf4 (100 ffu/cell) or were mock infected. The cells were visualized at the indicated times post infection (p.i.) using a Zeiss Axioskop at a magnification of 100. Mock infected samples are from the 24 hrs time point. Representative pictures of two independent experiments are shown. The percent of area covered with cells out of the total field area (ce) was determined using Image-Pro Premier 9.1 and the percent of area covered with cell clusters larger than 1000 pixels2 out of total cell area (cl), was calculated using FIJI. (B) Production of viruses in cells infected as described in A was determined by a titer assay. p.i.: post infection. Data is displayed in box plots as previously described [72]. Statistical significance was calculated using unpaired t-test and p values of significant differences between dl366* and dl366*+E4orf4 viruses are shown.