File S1 - Perivascular Delivery of Notch 1 siRNA Inhibits Injury-Induced Arterial Remodeling

Contains the following: Figure S1. Perivascular delivery of Notch 1 siRNA modulates PCNA expression in injury-induced vascular remodeling. Photomicrographs of confocal immunofluorescence (30X) staining for dual PCNA (Red)/α-Actin (Green) expression in carotid arteries 14 d after ligation in sham, ligated control (scrambled siRNA) and ligated Notch 1 siRNA vessels. Data are mean ± Sem. Figure S2: γ-secretase inhibition of Notch 1 IC Expression. Representative western blot of Notch 1 IC protein expression in HCASMC following γ-secretase inhibition with 20 µM DAPT for 24 h. Figure S3: Cytoplasmic Staining of Notch 1 IC in Medial/Intimal Smooth Muscle Cells. Photomicrographs of confocal immunofluorescence (30X) for dual staining for Notch 1/DAPI in ligated carotid artery 14 d after ligation. White arrows indicate cytoplasmic staining for Notch 1 IC around DAPI stained nuclei. Data are mean ± SEM, 4 sections analyzed/animal, n = 6 animals for each group. (PPTX)