Transcriptomic profiles of gene expression of brain tissue in duck in response to Tembusu virus infection

We used high-throughput RNA-sequencing technology to reveal the transcriptome profile of DTMUV-infected duck brain tissue. Overall design: Twenty-four 10-day-old SPF ducks (Harbin Veterinary Research Institute, Harbin, China) were randomly divided into two groups. The ducks were raised in separated negative-pressure isolators. The challenge group was intramuscularly inoculated with 0.5 ml of 105.5 TCID50/ ml TMUV JM strain. The control group was inoculated with an equal amount of sterile PBS. At 12, 24, and 48 hours post infection (hpi), four ducked were randomly selected from each group for euthanasia. The brain tissues were collected and pooled for NGS sequencing.