Holistic characterization of single hepatocyte transcriptome responses to high fat diet

Purpose: The goals of this study are to investigate the effects of the long-term high-fat diet to transcriptome of mouse hepatocytes utilizing single cell RNA-seq (scRNA-seq) technology. Methods: Hepatocytes were obtained from three mice that had been fed with the high-fat diet (HFD) and two mice that had been treated with the regular diet (LFD). After isolation and enrichment, hepatocytes were subjected to Drop-seq. Overall design: A total of 5 Drop-seq experiments (2 LFD and 3 HFD mice) were run. Sequencing was performed through Illumina HiSeq-4000 High-Output system.