Assessing the effect of Flubendazole on Jurkat T cells

Human Junket T cells grown to confluence in 100 mm plates in complete medium were incubated with 10 µM Flubendazole (FLU) or vehicle for 4 h. Total RNA was extracted using the RNeasy Mini Kit (Qiagen, cat. 74004) with minor modification. Samples were submitted to the UNSW Ramaciotti Centre for sequencing and gene expression analysis was carried out. Overall design: Comparative gene expression profiling analysis of RNA-seq data for Jurket T cells with and in the absence of FLU treatment.