Shoot proliferation and callus regeneration from nodular buds of Drepanostachyum luodianense
收藏国家林业和草原科学数据中心2022-10-31 更新2024-03-06 收录
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This research reports on an efficient shoot pro-liferation and callus regeneration system for bamboo.Young, semi-lignified branches with one lateral bud fromDrepanostachyum luodianense (Yi et R.S. Wang) Keng f.were used as explants.Disinfection with 0.1% HgCl2for8 min was the optimum treatment and the best medium forbud initiation was Murashige and Skoog (MS) mediumcontaining 3.0 mg LT' 6-benzyladenine (BA). Multipleshoots were induced from nodal shoot segments on MSmedium containing 5.0 mg L- BA,0.5 mg L-1 kinetin(Kin), and 1.0 mg L7' naphthaleneacetic acid (NAA).The highest frequency of callus formation (65.6%) was on MSmedium containing 4.0 mg L-' 2,4-dichlorophenoxyaceticacid (2,4-D),0.5 mg L-1 NAA,and 0.1 mg L-1 thidi-azuron (TDZ).
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国家林业和草原科学数据中心
创建时间：
2022-10-31
