Sequencing of fractionated marine nanoparticles from Station ALOHA (HOT319)

Seawater from 25m at Station ALOHA in the North Pacific Subtropical Gyre was filtered through a 0.2 µm filter and separated into extracellular vesicle-enriched and viral-like particle enriched fractions. Particle fractions were sequenced with an Oxford Nanopore R9.4.1 flowcell. Frameshifts introduced from Nanopore sequencing error were corrected using the proovframe algorithm (https://github.com/thackl/proovframe) and Illumina short-read sequences from the identical DNA samples.

This dataset includes all proovframe-corrected Nanopore sequences (>1kb) from the two fractions in FASTA format, each fraction divided into multiple gzip-compressed files:

EV_enriched_corrected_nanopore_[01-10]: reads from the extracellular vesicle-enriched fraction

VLP_enriched_corrected_nanopore_[01-10]: reads from the viral-like particle-enriched fraction

Additionally, we provide assembled metagenomic contigs from the cellular (>0.2µm) fraction of the same water sample in HOT319_25m_cellularfraction_assembly.fna.gz

The original raw seqence data used to generate these datasets can be obtained from the NCBI sequence read archive (Bioproject PRJNA855972).