Histone H2B.8 compacts flowering plant sperm through chromatin phase separation

We characterised a sperm-expressed histone variant, H2B.8 in Arabidopsis by performing ChIP-seq, RNA-seq, and Hi-C. The data show that H2B.8 is exclusively deposited to unexpressed euchromatin and that gene expression is not significantly affected either by h2b.8 mutation in sperm or overexpression in soma. The deposition of H2B.8increases the contact probability of short range intrachromosomal interactions and contacts between pericentomeric regions and chromosome arms of different chromosomes. ChIP-seq profiles of H2B.8 in sperm and ectopic H2B.8 in seedlings. Native ChIP-seq of H2B.8p::H2B.8-eGFP pollen and 35Sp::H2B.8-eGFP seedlings was used with GFP-Trap beads for immunoprecipitation. The ChIP-seq data includes two replicates of IP and respective inputs each. RNA-seq data comparing WT and h2b.8 mutant sperm cells and WT and H2B.8 ectopic expression (35Sp::H2B.8-eGFP) seedlings. The data includes two replicates of sperm cells and three replicates of seedlings for each. Hi-C data for WT and H2B.8 ectopic expression (35Sp::H2B.8-eGFP) seedlings.