The success rate of gene deletion using ca. 1.2 kb inverted repeat of individual gene.
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*The success rate was based on the PCR analysis of 10 colonies randomly selected from the selective plates. The correct integration was analyzed by PCR amplification with the confirmation oligos C1 and C2, and the excision rate was based on PCRs using the confirmation oligos C1 and C4.*In cases where the size of a targeted gene is below 1 kb, its flanking sequences together with the structure gene were amplified as a 1.2 kb inverted repeat.
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2015-12-02
