Siwi and BmAgo3 IPed piRNA profile in BmN4 cells

BmN4 cells, derived from silkworm ovaries, have both the ping-pong and phased piRNA pathways, making them valuable for piRNA research. Like silkworm ovaries, BmN4 cells express two PIWI proteins, Siwi and BmAgo3, which participate in the ping-pong amplification of piRNAs. Siwi preferentially associates with piRNAs with uracil at the first position (referred to as 1U bias), whereas BmAgo3-bound piRNAs show a bias toward adenine at the tenth position (referred to as 10A bias).