The endophytes of invasive clonal plant Wedelia trilobata
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Amplicon sequencing of endophytic bacteria and fungi of stems in invasive clonal plant Wedelia trilobata from four different geographical latitudes was obtained using Illumina Novaseq 6000 platform to obtain paired-end (PE) reads. Amplification of the V3–V4 region of bacterial rRNA genes was completed using the universal primers (341F: 5’- CCTACGGGNGGCWGCAG-3’; 806R: 5’-GGACTACHVGGGTWTCTAAT-3’). The ITS2 region of fungal rRNA genes was amplified with ITS1 and ITS2 primer (ITS1F: 5’- CTTGGTCATTTAGAGGAAGTAA-3’; ITS2R: 5’-TCCTCCGCTTATTGATATGC-3’). The endophytes of invasive clonal plant Wedelia trilobata were from four different geographical latitudes, (1) Wenzhou City (WZ: 27.919617N, 120.698494E), Zhejiang Province, (2) Quanzhou City (QZ: 24.901539N, 118.616325E), Fujian Province, (3) Zhanjiang City (ZhJ: 21.270611N, 110.351982E), Guangdong Province and (4) Sanya City (SY: 18.28169N, 109.508509E), Hainan Province.
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CNGB
创建时间:
2022-11-23



