Different iVα14 NKT subsets in total lymphocytes of thymus, spleen and liver in WT, B7-1/2 and CD28 deficient mice.

Lymphocytes from thymus, spleen and liver of age and sex-matched WT, B7-1(-/-)/B7-2(-/-) or CD28(-/-) mice were analyzed by flow cytometry. The iVα14 NKT cells were identified based on expressing TCRβ and binding to the α-Galcer/CD1d tetramer. Data shown are means and SD of the percentage of given subsets in total viable lymphocytes (n = 4). Similar data were obtained in another experiment involving 5 mice per group.*P**P***P