Modeling of the Response to a Multiple Vaccination Schedule Using the Adjuvant Neisseria meningitidis Porin B

Vaccine adjuvants enhance the immune response to vaccines, resulting more robust and durable immune responses. Although extensive work has been done on the response to a single vaccination over time, here we seek to characterize the response over the course of multiple vaccinations. In particular we modeled the changes in expression of mouse splenocytes after each of three vaccinations with and without antigen (chicken egg white ovalbumin) and adjuvant (N. meningitidis PorB). We also fitted the regulated genes into enriched gene sets to better characterize the response. 2x2 factorial design (+/- antigen, +/- adjuvant) over 3 time points, with a single universal control (PBS), n=3