Whole Exome Sequencing of heptocellular carcinomas from HBV1.3 mice.
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https://www.ncbi.nlm.nih.gov/sra/ERP158484
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DNA of micro-dissected liver tumor samples and matched control samples (non-tumor liver tissue) was extracted from 20-30 mg snap frozen tissue from HBV1.3 mice using the QIAamp DNA Mini Kit (Qiagen) according to manufacturer's recommendations. DNA concentration was determined by Qubit measurement (Qubit dsDNA BR Assay Kit, Thermo Fisher Scientific). Exome enrichment and sequencing was performed at the DKFZ Genomics and Proteomics Core Facility. Samples were sequenced on a NovaSeq 6000 platform (Illumina; Paired-End 100 bp S1 flow cell). Average read count was 68.219.436,17 (+/- 5.997.157,52) reads. For the analysis of somatic alterations in liver cancer samples obtained from HBV1.3 mice, a matched non-tumor liver sample was used as reference sample.
创建时间：
2024-07-28
