Massively Amplified Cell Traction Force Measurements using Cas12a

This dataset supports the publication Massively Amplified Cell Traction Force Measurements using Cas12a” - Duan et al., Nature Biomedical Engineering, 2023 Raw Fluorescence microscopy acquisitions of platelets, 3T3 Fibroblasts, Hela cells tension signal is included here. Each acquisition consists of images of the experiment labeled on name of different cells and fluorescent channels in a single nd2 file. To view images, open them in Nikon Elements software or in Fiji (or ImageJ with the bioformats plugin installed). The data shown here should include replicates obtained to ensure reproducibility. The entire folders were uploaded and contain acquisitions that were ultimately not included in analysis due to surface defects. Please consult with me if you have questions or ideas for how to use the data. Experiments conducted for measuring 3T3 cells’s tension signal at different cell numbers and inhibited by different concentration of platelets, or on different force threshold surface, platelets tension signal on different force threshold surface are stored in the main figure data folder. Imaging data supports the extended figures and supplementary figures including all the antiplatelets drug treated tension data, calibration tension data, platelet agonists tension data, MCATS optimization tension measurement data, different activator surface coverage data, different number of platelet’s tension data and lyophilized platelets tension data are stored in the supplementary and extended data folder.