Supplementary data and analysis code for: Melatonin-receptor dependence of the intestinal, metabolic and behavioural consequences of rest-phase restricted feeding in rats

Complete dataset and analysis code for the manuscript "Melatonin-receptor dependence of the intestinal, metabolic and behavioural consequences of rest-phase restricted feeding in rats" by Mohamed E. Elbeeh (Department of Biology, Jamoum University College, Umm Al-Qura University, 21955 Makkah, Saudi Arabia). STUDY. Sixty-six male Wistar rats were randomised to seven arms and followed for 28 days: dark-phase (active-phase) restricted feeding; light-phase (rest-phase) restricted feeding; and rest-phase feeding with melatonin, with melatonin plus luzindole, or with luzindole alone, together with a drug-alone dark-fed control and a pair-fed subgroup. Melatonin (10 mg/kg) and luzindole (20 mg/kg) were given intraperitoneally at ZT12 in 0.5% ethanol in saline. Terminal procedures were performed at ZT4 or at ZT16, assigned at randomisation. Ethics approval HAPO-02-K-012-2024-01-2702. The study was not prospectively registered. ENDPOINTS. The primary endpoint was in-vivo intestinal permeability, measured as plasma fluorescein isothiocyanate-dextran of 4 kDa. Secondary endpoints covered food and water intake and body-weight trajectory, oral glucose and insulin tolerance with HOMA-IR, home-cage activity, six behavioural tasks, plasma melatonin and corticosterone at both zeitgeber times, colonic and hippocampal gene expression by RT-qPCR, tissue redox and monoamine measures, and isolated distal-colon contractility to carbachol. CONTENTS. DataTemplate_FILLED_P02.xlsx is the source workbook and holds every raw reading, including plate fluorescence and standard curves, Cq values, organ-bath traces and the full animal log. analysis_restphase_feeding_melatonin_v2.py is the pre-specified analysis script, fixed before the data were analysed; stats_full.json and analysis_log.txt are its complete output and run log. audit.py recomputes the headline results independently from the raw plate readings and Cq values and compares them with the manuscript. verify_primers_v2.py performs in-silico PCR for all twenty primer pairs against the Rattus norvegicus RefSeq transcripts they target, and primer_check_v2.json is its output. Supporting_scripts_and_result_tables.zip contains the remaining scripts and result tables 7 to 14. Supplementary Tables S2 and S8 give the MIQE primer table and the assay reagent lots. ACCESS. Files are restricted. Access is granted on reasonable request to the depositor for non-commercial academic use, consistent with the data-availability statement of the manuscript.