Path-seq identifies essential mycolate remodeling program for mycobacterial adaptation in host cells [RNA-seq]

Comparison of transcriptional differences (RNA-seq) between induced and uninduced overexpression of MSMEG_0916 in Mycobacterium smegmatis Cultures of Mycobacterium smegmatis with pDTCF-MSMEG_0916 were grown in the presence or absence of anhydrotetracycline (ATc) for 4 hour and harvested for mRNA expression profiling by Illumina sequencing, in triplicate. The sequence reads that passed quality filters were aligned with Bowtie2 and processed using the R package DuffyNGS.