Suplementary information of the work entitled “Genomic insights into the evolution and adaptation of secondary metabolite gene clusters in fungicolous species <i>Cladobotryum mycophilum</i> ATHUM6906”, authored by Anastasia C. Christinaki, Antonis I. Myridakis and Vassili N. Kouvelis*.*corresponding author

Legends of supplementary materials File S1. Transposable elements identified in C. mycophilum ATHUM6906 genome using RepeatModeler. File S2. Orthogroups among selected species representing Hypocreaceae family, i.e., T. harzianum, T. virens, C. dendroides, C. protrusum, Escovopsis sp. and H. perniciosus, with the entomopathogenic species B. bassiana used as outgroup (NCBI assembly accessions: GCF_003025095.1; GCF_000170995.1; GCA_011799845.1; GCA_004303015.1; GCA_003055185.1; GCA_008477525.1; GCA_000280675.1, respectively), as identified using the OrthoFinder algorithm. File S3. Schematic representation of siderophore BGCs in Cladobotryum genomes and the phylogenetic tree produced by the ML method in IQ-TREE. In the phylogenetic tree siderophore core genes belonging to Cladobotryum genomes are highlighted with red color. Bootstrap values are indicated in the nodes. File S4. Schematic representation of T3PKS BGCs in Cladobotryum genomes and the phylogenetic tree produced by the ML method in IQ-TREE. In the phylogenetic tree T3PKS core genes belonging to Cladobotryum genomes are highlighted with red color. Bootstrap values are indicated in the nodes. File S5. Schematic representation of NAPAA BGCs in Cladobotryum genomes and the phylogenetic tree produced by the ML method in IQ-TREE. In the phylogenetic tree NAPAA core genes belonging to Cladobotryum genomes are highlighted with red color. Bootstrap values are indicated in the nodes. Figure S1. GO annotation of the expanded and contracted gene families in C. mycophilum compared to selected species representing Hypocreaceae family, i.e., T. harzianum, T. virens, C. dendroides, C. protrusum, Escovopsis sp. and H. perniciosus, with the entomopathogenic species B. bassiana used as outgroup (NCBI assembly accessions: GCF_003025095.1; GCF_000170995.1; GCA_011799845.1; GCA_004303015.1; GCA_003055185.1; GCA_008477525.1; GCA_000280675.1, respectively). Figure S2. Network analysis of the BGCs located in 40 Hypocreaceae genomes. Table S1. Annotation of C. mycophilum identified PCGs against InterPro, Swissprot, PDB, KEGG, CAZy, PHI, COG, tcdb and TMHMM databases. Each annotation is presented in a different sheet which is named after the respected database. Table S2. PCGs related to chitin synthesis and degradation, their annotation description, the CAZy family the belong and the identification of the secretion signal peptide (Sec/SPI) whenever found. Table S3. PCGs related to glucan degradation, their annotation description, the CAZy family the belong and the identification of the secretion signal peptide (Sec/SPI) whenever found. Table S4. Predicted secretory PCGs in C. mycophilum genome and their classification against PHI, CAZy, TCdb and MEROPS databases, along with the PredGPI prediction server. Table S5. Pathogenicity-related genes encoded transporters according to PHI and tcdb databases. Table S6. The identified BGCs, their type, their locations in C. mycophilum genome and their similarity (%) with the most known secondary metabolic clusters. Table S7. The overall and the species specific statistics of the orthologous comparative analysis of Hypocreaceae secondary metabolism. Table S8. Secondary metabolism-related orthogroups found only in Cladobotryum species, their characterization and their mapping against the GO database. Table S9. Alien Index analysis of the PCGs belonging to the BGCs of category C and the terpene BGC of Basidiomycetous origin.