RNA-seq profiling of adult and late third instar larval testes from Drosophila melanogaster males and translocationsT(1;2) males

In order to study the effect of translocation T(1;2) on gene expression in testes, we performed polyA+ RNA-seq profiles from pools of adult and third instar larval testes from 7 translocation lines in biological quadruplicate. Among the 7 translocations, 5 are male sterile, 2 are fertile. In order to compare the expression profiles of translocations to the control, we sequence the testes from the parental flies that were irradiated to generate the translocation lines. We prepared 50bp stranded single end read libraries. Overall design: We collected the dissected late third instar larval (L3) testes and one-to-five-day adult fly testes. We pooled 15 L3 testes and 20 adult testes for each sample (see "Extraction protocol" for details). Even though all the samples were collected on different days and stored at -80ºC until the RNA extraction, we extracted RNA and prepared the library simultaneously for all the samples in a 96 well plate.