Supporting data for "The Tumour-Suppressive Role of GPR15L and Its Interaction with GPR15 in Colorectal Cancer"

The study of GPR15L and GPR15 and their interaction in CRC. Functional assays, including proliferation, drug resistance, colony formation, tumour spheroid formation, and migration assay, were done to investigate the outcome of modulating expression of either GPR15L and GPR15, as well as treatment of cells with secreted GPR15L. In vivo study of GPR15L overexpression in tumour cells subcutaneously injected into immunodeficient mice was used to identify its tumour-suppressive effect. Correlations between GPR15L expression in CRC or normal adjacent tissues and patient clinical characteristics were identified. Western blot was performed to analyse the change in protein expression upon change in GPR15L and GPR15 expression.

本研究旨在探讨GPR15L和GPR15及其在结直肠癌（CRC）中的相互作用。通过对GPR15L和GPR15表达水平的调控，以及用分泌的GPR15L处理细胞，进行包括增殖、药物耐药性、集落形成、肿瘤球形成和迁移实验在内的功能实验，以探究这些调控措施对细胞的影响。此外，通过在免疫缺陷小鼠皮下注射肿瘤细胞并研究GPR15L过表达的现象，以识别其抑瘤作用。同时，确定了CRC或正常邻近组织中GPR15L表达与患者临床特征之间的相关性。通过Western blot技术分析了GPR15L和GPR15表达水平变化时蛋白质表达的改变。