GNPS-UTEP_Chagas disease vaccine_Baboon heart metabolomics_June 2017

Baboons were immunized with L-MPLA adjuvant only, or adjuvant+KM24h, +MASPpep, or +KM24h&MASPpep combination. Animals were challenged twice with Trypanosoma cruzi. Hearts were collected 168 days post-infection. Ventricles were divided and sectioned. Metabolites were extracted by a two-step protocol, first with 50% methanol (aqueous extract) then with 3:1 dichloromethane:methanol (organic extract). Liquid chromatography separation was performed on a Kinetex C8 LC Column 50 x 2.1 mm, with water+0.1% formic acid and acetonitrile+0.1% formic acid as mobile phases.