Illumina sequencing raw data for "Quantification of total fish eDNA metabarcoding concentration to optimize the detection of rare species and sampling effort"

The dataset contains the following files: Sequencing information for each sample, in a table that contains: LIBRARY: library where the samples were sequenced. SAMPLE: sample code, with a suffix "_" indicating the PCR replicate number for each sample. TAG: DNA barcode used for demultiplexing. PRIMER F / PRIMER R: forward/reverse primer sequences (Teleo primers). Run MB0622M: raw sequencing files for the 11 libraries, each provided as two fastq.gz files: forward (LIBRARY_R1.fastq.gz) and reverse (LIBRARY_R2.fastq.gz). The samples used in this study are the samples having a code starting with "SPY" in the SAMPLE column. Samples having a "CNEG" value correspond to negative control samples. Samples having an "OTHER" value do not concern this study, but should be analysed in parallel to filter tag-jump in each libraries. Samples having a "BLANK" value correspond to tag combinations that were not assigned to any sample within a given library; they should likewise be analysed in parallel to calculate index-hopping between libraries. The detailed methods explaining how this dataset was collected and analysed can be found in the methods section of Didier Pont, Paul Meulenbroek, Maxime Logez, Andrea Funk, Alice Valentini, Tibor Erős, Thomas Hein (submitted). Quantification of total fish eDNA metabarcoding concentration to optimize the detection of rare species and sampling effort. Environmental DNA