Minimum requirements and brief characterization of three tested library preparation kits.
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The minimum requirements according to manufacturers' protocols are the quality of pre-depletion RNA and the amount of input rRNA-depleted RNA. The quality of RNA is based either on RNA integrity number (RIN) or the source of RNA, for example formalin-fixed paraffin-embedded (FFPE) tissue blocks. Preceding depletion of rRNA is required for all library prepration procedures.
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2019-02-11
