genomic and transcriptional variations in rapeseed genotypes with contrasting boron efficiencies

An Illumina HiSeq 2000 system (read length = 100 bp) (Illumina Inc., San Diego, CA, USA) was used to perform WGS to distinguish the genomic variations (including single nucleotide polymorphisms, SNPs and insertions/deletions, InDels) between ‘QY10’ and ‘W10’, which generated a total of 40.0 Gb data.The leaves and roots of ‘QY10’ and ‘W10’ seedlings exposed to B deficiency for 10 days were subjected to DGE profiling. The total RNA of each sample was subsequently sequenced on an Illumina Hiseq 2500 platform (San Diego, CA, USA) to generate 50-bp single-end (SE) reads.