Blood gene expression profiles following influenza vaccination in pregnant women

Influenza immunization during pregnancy provides protection to the mother and the infant. Studies in adults and children with inactivated influenza vaccine (IIV) have identified changes in immune gene expression that correlated with antibody responses. We studied changes in transcriptional profiles induced by IIV in pregnant women and to identify correlates of antibody responses. Whole blood was collected in Tempus tubes from 44 pregnant women on day 0 (pre-vaccination), day 1 and day 7 post-vaccination. RNA was extracted and globin reduced. Labeled cRNA was hybridized to Illumina Human HT-12 Beadchips.