Comparison of asexual and sexual growth among <i>PsMYB1</i>-silenced and non-silenced strains.

aStrains are the P6497 wild-type (parental) or transformants derived from P6497 (by protoplast transformation). Independent-samples t-test between wild-type P6497 and each mutant was performed and marked by "*" and "**", referring to p<0.05 and p<0.001, respectively. Values in the table are the mean ± standard error. bBased on 5 days of growth in 10% V8 juice agar medium. cNumber of 5×5 mm colonies releasing zoospores after washing for 24 h at 25°C. The numbers of zoospores in 10 ul of sterile distilled water were counted. dPercent of encysted zoospores in 50 ul of zoospores suspension after 1 h incubation at 25°C with 80% humidity. ePercent of cysts forming germ tubes after 2 h incubation with vortexing to induce encystment, based on counting a minimum of 100 cysts from each strain. fNumber of oospores was counted in 10% V8 juice medium for 10 days.