Kinetic constants for wt Mtb Pck in the presence of different divalent cations.

aFor gluconeogenic reaction 100 mM HEPES-NaOH, pH 7.2, 0.3 mM OAA, 0.2 mM GTP, 2 mM MgCl2, 0.2 mM MnCl2, 10 mM DTT, 10 U/ml LDH, 3 U/ml PK and 0.2 mM NADH was used.bFor anaplerotic reaction 100 mM HEPES-NaOH, pH 7.2, 100 mM KHCO3, 37 mM DTT, 2 mM PEP, 1 mM GDP, 2 mM MgCl2, 0.1 mM MnCl2, 2 U/ml MDH and 0.25 mM NADH was used.n.d.: Kinetic constants cannot be calculated.Kinetic constants for wt Mtb Pck in the presence of different divalent cations.