To investigate the downstream target gene of YAP1 in enzalutamide-resistant (EnzaR) cells and the effects of extracellular vehicles (EVs) derived from EnzaR cells on enzalutamide-sensitive cells by next-generation sequencing analyses

Our study has identified that yes-associated protein 1 (YAP1) is overexpressed in enzalutamide-resistant (EnzaR) cells. Furthermore, enzalutamide-induced YAP1 expression is mediated through the function of chicken ovalbumin upstream promoter transcription factor 2 (COUP-TFII) at the transcriptional and the post-transcriptional levels. Furthermore, both YAP1 and COUP-TFII have proven to be cargoes of EVs by our study. Next, treatment with EnzaR-EVs induces the abilities of enzalutamide resistance in its parental cells while depletion of YAP1 or COUP-TFII in EnzaR-EVs attenuated it. In order to systemically investigate the downstream target gene of YAP1 and the expression profiles changed by EnzaR-EVs from control, YAP1 and COUP-TFII depletions in prostate cancer cells, we have performed RNA-seq analyses to pursuit the research directions of those questions. RNA samples were isolated from EnzaR cells treated with siRNA against YAP1 and control siRNA (n=2), and from LNCaP cells treated without or with control, YAP1- and COUP-TFII-depleted EnzaR-EVs (n=1) for 48 hours.