Effects of ectopic expression of pik1 alleles on cytokinesis in cdc4 cells.
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Cells were cultured at 30°C for 24 hours, + or − thiamine. Cell length at division (cells with septa) was measured and expressed as mean±S.E., n = 25–50 cells. Contractile ring indices were estimated as the proportion of the cells with medial bands containing F-actin (CR-A) or myosin (CR-M), as visualized with FITC-phalloidin or indirect immunofluorescence, respectively. Similarly, septum indices were estimated with calcofluor-white (Septa), and the frequencies of binucleate cells with DAPI (BiN). n = 100–450 cells.
创建时间：
2015-12-02
