Comparison of CRISPR-Cas9 genome-edited JEG-3 TAZ/WWTR1 knockout and wild type choriocarcinoma cells

The transcriptional co-activator TAZ/WWTR1 plays a central role in the Hippo signaling pathway and acts as crucial mediator in maintaining organ size and tissue homeostasis. Changes in its activity can lead to diseases, including cancer, due to uncontrolled cell growth or aberrant cellular behavior. The aim of this study was to identify gene signatures that are specifically regulated by TAZ in human trophoblast cells. This was achieved by comparing JEG-3 wild-type and TAZ knockout choriocarcinoma cells, generated with CRISPR-Cas9 technology. Overall design: The JEG-3 TAZ wild-type and knockout cell lines were generated using CRISPR-Cas9 genome-editing, resulting in two wild-type and two TAZ knockout lines. Next-Generation Sequencing was employed to analyze the gene expression differences across 8 samples, comprising the two wild-type cell lines and the two knockout cell lines, each measured in duplicates.