Nucleotide sequences of the primers used in this study.
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Y = c+t; R = a+g, U = dUTP; In Alu-PCR, four sets of PCR were performed using Alu3/PCFo, Alu3/PCRo, Alu5/PCFo, and Alu5/PCRo as the first stage PCR primers. Subsequently, uracil DNA glycosylase was added to break the DNA strands at apurinic dUTP sites. Finally, Tag3/PCFi, Tag3/PCRi, Tag5/PCFi, and Tag5/PCRi were used respectively for the second stage PCR.
创建时间：
2015-12-02
