Lipidomics Methods Lipid extraction: HEK293T cells were seeded in 6-well plates, grown to 70% confluency and treated with bumped fatty acid probes (15 µM) for 4 h. Cells were dislodged i
Scatter plot of PCA including the proportions of all identified fatty acids in the non-fractionated extract and 4 lipid fractions of serum and follicular fluid
Multiple Reaction Monitoring (MRM) analysis of lipid extracts from purified GRCR.Shin, J. et al. Constitutive activation mechanism of a class C GPCR. Nat. Struct. Mol. Biol. 1–10 (2024) doi:10.1038/s4
Lipid, an important organic component in milk, has health benefits for newborns. Profiles of milk lipid in multi-species from multi-level haven’t been accessible, therefore, we established a lipid sub